Lyophilized solid. Potent and specific inhibitor of multiple ubiquitin hydrolases involved in pathways of intracellular protein modification and turnover. Useful for stabilizing endogenous or in vitro synthesized ubiquitin-protein conjugates. May also be used to enhance or decrease rates of ubiquitin-dependent degradation. Biological activity: Shown to inhibit the activity of Isopeptidase T (Cat. No. 419700) and Ubiquitin C-Terminal Hydrolase (Cat. No. 662090). Purity: ≥95% by HPLC. Ref.: Melandri, F., et al. 1996. Biochemistry 35, 12893. Shaeffer, J.R. and Cohen, R.E. 1996. Biochemistry 35, 10886. Pickart, C.M. and Rose, I.A. 1986. J. Biol. Chem. 261, 10210. |
