Liquid. In 50 mM HEPES, pH 8.0. AVOID FREEZE/THAW CYCLES. Native, rabbit ubiquitin-activating enzyme E1. Catalyzes the first step in the ubiquitin-protein isopeptide bond formation. Activates ubiquitin monomer at its C-terminal cysteine residue by forming a high energy thioester bond. This activated ubiquitin is then transferred to lysine residues on target proteins via the E2/E3 conjugation cascade. Critical component for the initiation of conjugation reactions in vitro. Purity: ≥95% by SDS-PAGE. Ref.: Tanaka, K. 1998. J. Biochem. (Tokyo) 123, 195. Ciechanover, A., et al. 1982. J. Biol. Chem. 257, 2537. Haas, A.L., and Rose, I.A. 1982. J. Biol. Chem. 257, 10329. Ciechanover, A., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 761. |
