Sentrin/SUMO-Activating Enzyme Protein AOS1/UBA2
Smt3 Activating Enzyme
SUMO-1 Activating Enzyme Subunit 1
SUMO-1 Activating Enzyme E1 N subunit
Ubiquitin-like Protein SUMO-1 Activating Enzyme

Liquid. In 150 mM NaCl, 75 mM Tris-HCl, 5 mM DTT, 20% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human SUMO-activating enzyme E1 subunits, Aos1 and Uba2, each fused at the N-terminus to a His•Tag^® sequence and expressed in S. frugiperda insect cells. SUMO-activating enzyme is a heterodimer composed of Uba2 and Aos1 polypeptides, which resembles ubiquitin in its structure, its ability to be ligated to other proteins, as well as in the mechanism of ligation. However, SUMOlation does not result in proteasome-mediated protein degradation. It mediates ATP-dependent activation of UBL1 (ubiquitin-like 1) and formation of a thiolester with a conserved cysteine residue on SAE2. Migrates as two bands by SDS-PAGE, the ~ 70 kDa Aos1 GST-fusion protein and the ~ 110 kDa Uba2 GST-fusion protein. Also available as a non-fusion protein under Cat. No. 662074. Purity: ≥98% by SDS-PAGE.

Ref.: Tatham, M.H., et al. 2001. J. Biol. Chem. 276, 35368. Gong, L., et al. 1999. FEBS Lett. 448, 185. Okuma, T., et al. 1999. Biochem. Biophys. Res. Commun. 254, 693. Johnson, E.S., et al. 1997 EMBO J. 16, 5509. Dohmen, R.J., et al. 1995. J. Biol. Chem. 270, 18099.