Diubiquitin Liquid. In 150 mM NaCl, 20 mM HEPES, 1 mM DTT, 10% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human FAT10 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. FAT10 is a small ubiquitin-like modifier protein whose expression is synergistically inducible with g-interferon and TNF-a. It is composed of two ubiquitin-like domains and bears a di-glycine motif at the C-terminus. The wild-type form of FAT10 (but not the di-glycine form) has been shown to conjugate to target proteins. FAT10 expression also causes apoptosis. Purity: ≥95% by SDS-PAGE. Ref.: Hipp, M.S., et al. 2004. J. Biol. Chem. 279, 16503. Lee, C.G., et al. 2003. Oncogene 22, 2592. Raasi, S., et al. 2001. J. Biol. Chem. 276, 35334. Liu, Y.C., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 4313. |