Liquid. In PBS. AVOID FREEZE/THAW CYCLES. Recombinant, human, UbcH8 conjugating enzyme fused to a His•Tag^® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. UbcH8 is a member of the E2 family of ubiquitin conjugating enzymes and resembles UbcH7 instructure. It is specific for the conjugation of interferon-stimulated gene 15 (ISG15) to a variety of target proteins follwoing stimulation with IFN-a or IFN-b. ISG15 conjugation then targets proteins for proteosomal degradation. In addition to ISG15, UbcH8 is known to associated with the Parkin protein and Staring, both of which are characterized as an E3 ubiquitin ligases. Binding to Parkin results in the activation of its ubiquitin ligase activity. Staring, which interacts with syntaxin-1, recruits UbcH8 to syntaxin-1, facilitating its ubiquitination and proteasome-mediated degradation. Purity: ≥95% by SDS-PAGE.

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