(His)6 UbcH10 (E2) Liquid. In 125 mM NaCl, 50 mM HEPES, 1 mM DTT, 10% glycerol, pH 8.0. AVOID FREEZE/THAW CYCLES. Recombinant, human ubiquitin conjugating enzyme 10 fused to a His•Tag® sequence and expressed in E. coli. E2s are a family of ubiquitin carrier proteins that transfer ubiquitin to the e-lysine of proteins designated for degradation. UbcH10 in conjunction with the anaphase-promoting complex catalyzes the destruction of cyclin A and cyclin B, thus playing an important role in the control of the cells exit from mitosis. Purity: ≥95% by SDS-PAGE. Ref.: Townsley, F.M., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2362. Varshavsky, A. 1997. Trends Biochem. Sci. 22, 383. Hochstrasser, M. 1996. Annu. Rev. Genet. 30, 405. Ciechanover, A. 1994. Cell 79, 13. Seufert, W., and Jentsch, S. 1990. EMBO J. 9, 543. |
