Liquid. Each enzyme is supplied in 50 mM HEPES, 50 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.8. AVOID FREEZE/THAW CYCLES. Contains 10 µg each of the following ubiquitin conjugating (E2) enzymes: GST-Fusion UbcH2 (Cat. No. 662111), His•Tag® UbcH3, GST-Fusion UbcH5a (Cat. No. 662091), GST-Fusion UbcH5b (Cat. No. 662092), His•Tag® UbcH6 (Cat. No. 662094), UbcH7, and His•Tag® UbcH10 (Cat. No. 662095). Useful for selecting the appropriate enzyme in novel reactions. Purity: ≥95% by SDS-PAGE. Ref.: Lin, Y., et al. 2002. J. Biol. Chem. 277, 21913. Hatakeyama, S., et al. 2001. J. Biol. Chem. 276, 33111. Pickart, C.M. 2001. Annu. Rev. Biochem. 70, 503. Zheng, N., et al. 2000. Cell 102, 533. Gonen, H., et al. 1999. J. Biol. Chem. 274, 14823. |
