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 | 2 to 8°C |  | Blue ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| | Ubiquitinated Protein Enrichment Kit |
|  | Cat. No. 662200 |
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| Form: 1 kit sufficient to process 12.5-25 mg lysate | | Kit Contents: Polyubiquitin Affinity Beads, Control Glutathione-Agarose Beads, Control Lysate, and a user protocol. | | Comments: A rapid method for isolating ubiquitinated proteins using affinity beads comprised of a GST-fusion protein containing an ubiquitin-associated sequence bound to glutathione-agarose. Useful for the enrichment of polyubiquitinated proteins from cell and tissue lysates of a broad range of species including canine, human, mouse, and yeast. The ubiquitinated proteins can be identified by loading the beads directly onto SDS-PAGE and then immunoblotting with the antibody of choice or Anti-Ubiquitin (Cat. No. 662099). Alternatively, it is possible that the beads can be treated with Isopeptidase T (Cat. No. 419700) to release the proteins from the ubiquitin chains. | | Ref.: Chen, L. and Madura, K. 2002. Mol. Cell Biol. 22, 4902. Chen, L., et al. 2001. EMBO Rep. 2, 933. | | R: 23/24-28-32-42-50/53; S: 26-27-28-36/37/39-45-60-61 | | Sold under license of PCT Application wo 03/049,602. |
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EMD Chemicals Inc. EUR list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| - Naoto Inukai, et al. (2004) A novel hydrogen peroxide-induced phosphorylation and ubiquitination pathway leading to RNA polymerase II proteolysis. Journal of Biological Chemistry 279, 8190-8195.
- Zhigang Zhang, Jin-Ying Wu, William Hait and Jin-Ming Yang. (2004) Regulation of the stability of P-glycoprotein by ubiquitination.. Molecular Pharmacology 66, 395-403.
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