Insulin Receptor Substrate-1 p30, Human, Recombinant, E. coli

Liquid. In 150 mM Tris-HCl, 50 mM HEPES, 1 mM DTT, pH 7.4. AVOID FREEZE/THAW CYCLES. Recombinant, human insulin receptor substrate 1 (IRS1) consisting of the p30 fragment (IRS1p30, amino acids 516-777) expressed in E. coli. IRS1 is a major endogenous substrate for insulin receptor and IGF-1R tyrosine kinase activity. Although IRS-1 is constitutively phosphorylated on given serine and threonine residues, it is tyrosine-phosphorylated only after stimulation by insulin. This recombinant p30 fragment contains 5 potential tyrosine phosphorylation sites; phosphorylated IRS1p30 will bind to insulin receptor. Purity: >95% by SDS-PAGE.

Ref.: Yong, W., et al. 2004. Acta Physiol. Sinica 56, 539. Siemeister, et al. 1995. J. Biol. Chem. 270, 4870.