Vascular Endothelial Growth Factor121 Lyophilized. Recombinant, human VEGF121 (121-amino acid splice variant of the human vascular endothelial growth factor VEGF) expressed in S. frugiperda insect cells. VEGF121 is dominantly expressed in most tumor cell lines. Forms a biologically active homodimer and evidence suggests that it is also biologically active as a heterodimer with other VEGF splice variants or PIGF. Biological activity: ED50 ≥1 ng/ml as measured by the stimulation of 3H-thymidine incorporation by human umbilical vein endothelial cells and bovine aortic endothelial cells. In vivo effects using the 13-day-old chick chorioallantoic membrane (CAM) assay can be seen between 0.5-4 µg. Purity: ≥90% by HPLC. Contaminants: Endotoxin: ≤0.1 ng/µg prtoein. Ref.: Stimpfl, M., et al. 2002. Clin. Cancer Res. 8, 2253. Pufe, T., et al. 2001. Arthritis Rheum. 44, 1082. Wilting, J., et al. 1996. Dev. Biol. 176, 76. Conn, G. et al. 1990. Proc. Natl. Acad. Sci. USA 87, 1323. |
