Vascular Endothelial Growth Factor164 Lyophilized solid. Recombinant, mouse VEGF164 (164-amino acid splice variant of VEGF) expressed in S. frugiperda insect cells. The most abundant form in mice, VEGF164 is a potent angiogenic factor and vascular permeability factor in vivo, as well as a chemoattractant for monocytes and endothelial cells. Forms a biologically active homodimer and evidence suggests that it is also biologically active as a heterodimer with other VEGF splice variants. Expression of VEGF164 appears to be essential for normal bone development and vascularization. Biological activity: ED50 ≥1 ng/ml as measured by the stimulation of 3H-thymidine incorporation by human umbilical vein endothelial cells and bovine aortic endothelial cells. In vivo effects using the 13-day-old chick chorioallantoic membrane (CAM) assay can be seen between 0.5-4 µg. Purity: ≥95% by SDS-PAGE. Ref.: Maes, C., et al. 2002. Mech. Dev. 111, 61. Grunstein, J., et al. 2000. Mol. Cell Biol. 20, 7282. Springer, M.L., et al. 2000. J. Gene Med. 2, 279. |
