Vascular Endothelial Growth Factor-C Lyophilized. Recombinant, rat VEGF-C (129-amino acids lacking the N- and C-terminal extensions and containing only the middle third VEGF homology domain) expressed in S. frugiperda insect cells. Forms primarily non-covalent linked dimers that can phosphorylate VEGF-R3 (Flt-4). Its actions are mediated via VEGFR-2 on vascular endothelial cells. VEGF-C has been shown to be involved in the regulation of growth and differentiation of lymphatic endothelium. Biological activity: ED50 ≥200 ng/ml as measured by the stimulation of VEGFR-3 phosphorylation in porcine endothelial cells. Purity: ≥90% by SDS-PAGE. Contaminants: Endotoxin: <0.1 ng/µg VEGF-C. Supplemented with 0.1% BSA or HSA. Ref.: Amioka, T., et al. 2002. Eur. J. Cancer 38, 1413. Kirkin, V., et al. 2001. Eur. J. Biochem. 268, 5530. Mandriota, S.J., et al. 2001. EMBO J. 20, 672. Karkkainen, M.J., and Petrova, T.V. 2000. Oncogene 19, 5598. Partanen, T.A., et al. 2000. FASEB J. 14, 2087. Joukov, V., et al. 1997. EMBO J. 16, 3898. |
