Rapid, quantitative precipitation of DNA and RNA, including PCR clean-up

Pellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (McCormick 1995, McCormick 1996). The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.

Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated (Taggart 1998). Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.

Pellet Paint Co-Precipitant is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package) can be used as a correction factor when determining nucleic acid concentration (McCormick 1996). Pellet Paint Co-Precipitant is compatible with automatted Cy5® sequencers. Pellet Paint NF Co- Precipitant is recommended for use with PE Applied Biosystems automated sequencers.

Features and Benefits:
• Direct visualization and tracking of precipitated material

References:
McCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.

Note: Euorpean Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending.

Components:

•	250 µl or 2 ml	Pellet Paint Co-Precipitant
•	1 ml or 8 ml	3 M Sodium Acetate, pH 5.2