Versatile plasmid for enhancement of in vitro translation, plus lac-driven E. coli expression The pT7Blue-2 Vector is a versatile plasmid featuring the 43-bp Xenopus b-globin 5′-UTR for enhancement of translation in rabbit reticulocyte lysates and frog oocytes (Krieg 1984, Falcone 1991). Inserts cloned in-frame with the lacZ a-peptide coding sequence can also be expressed in E. coli from the lac promoter. The vector has many additional features that make it an excellent choice for a variety of applications.
Features and Benefits:
- T7 promoter upstream of 5′-UTR plus optimal eukaryotic translation initiation site for efficient transcription/translation analysis with the Single Tube Protein® System 3, T7
- lac promoter-driven expression in E. coli; compatible with EcoPro™ coupled transcription/translation system
- Blue/white screening of recombinants (requires that in-frame inserts contain a translation termination codon)
- N-terminal 15-aa S•Tag™ fusion for quantitative detection and purification
- Available as a Perfectly Blunt® Vector Kit containing linearized, dephosphorylated pT7Blue-2 for convenient, efficient cloning of PCR products
- SP6 promoter adjacent to EcoR I cloning site enables in vitro synthesis of RNA with minimal extraneous sequence
- Enterokinase cleavage site enables removal of all vector-encoded fusion sequences
- Extensive multiple cloning region
- High-copy replicon for high plasmid yields and phage f1 origin for single-stranded DNA production
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