For random double-stranded cleavage of DNA

DNase I, ds Qualified is a special preparation of bovine pancreatic DNase I that can be used for double-stranded cleavage of DNA molecules in preparation for cloning or other applications. In the presence of Mn²⁺ and absence of Mg²⁺, the enzyme makes double-strand breaks at random sites in DNA. The average size of the resulting fragments can be controlled by the enzyme concentration and incubation time. This method is useful for the creation of shotgun libraries and is used in the NovaTope® System to prepare random subclones of target genes for functional domain analysis. The enzyme also is included in the DNase Shotgun® Cleavage Kit, which contains reaction buffer and MnCl₂ solution. For applications requiring general cleavage or degradation of DNA, please see RNase-free DNase I and Benzonase® Nuclease.

Unit Activity: One unit will degrade 1 µg DNA in 10 minutes at 37°C. The 50-µl reaction mixture contains 80 mM HEPES, pH 7.5, 10 mM NaCl, 5 mM MgCl₂, 10 mM DTT, 1 µg plasmid DNA, and enzyme.