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  AP Detection Reagent Kit
Cat. No. 69264  
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Detection » AP & HRP Blot Development Substrates
AP & HRP Blot Development Substrates
Optimal performance and convenience for Western and dot blot applications
 
The quality of the substrates used for signal development is critical to achieve the required sensitivity and low background in Western and dot blots. The substrates available here are tested for compatibility and reproducibility with all of our detection kits and components.

The AP Detection Reagent Kit includes standardized solutions of 3-bromo-4-chloro-5-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT), plus 20X AP Buffer, for sensitive chromogenic detection of alkaline phosphatase conjugates. With the NBT/BCIP system, positive bands turn a deep blue-violet color that resists fading.

For very high sensitivity, chemiluminescent detection is recommended. Both the CDP-Star® AP Substrate and SuperSignal® HRP Substrate enable subnanogram sensitivity in a convenient ready-to-use format. The CDP-Star Substrate also includes Nitro-Block-II™ signal enhancer for increased signal-to-noise ratios with standard nitrocellulose membranes. 
 
Product Description

The AP Detection Reagent Kit includes standardized solutions of 3-bromo-4-chloro-5-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT), plus 20X AP Buffer, for sensitive chromogenic detection of alkaline phosphatase conjugates. With the NBT/BCIP system, positive bands turn a deep blue-violet color that resists fading.

Components:
1 kit5 × 1 kitComponent
1.5 ml5 × 1.5 mlBCIP
1.5 ml5 × 1.5 mlNBT
20 ml5 × 20 ml20X AP Buffer
 

Need additional information about this product? Email our Technical Service department at: novatech@novagen.com

 Related information for this product is available:

EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Cat. No.
Size
In Stock
Qty
Select Country Above
For Pricing Information
69264-31 eaY$69.00
69264-45 ea*$263.00

* Additional Product Information
Cat. No.Disclaimers
69264-4
Stock StatusPlease contact our Customer Service Department for delivery time.

EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Product Name
Cat. No.
Size
In Stock
Qty
Select Country Above
For Pricing Information
SuperSignal® HRP Substrate69059-32 × 25 mlY$78.00
CDP-Star® AP Substrate69086-340 mlY$139.00


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69246: lBlueSTAR™ DNA
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Available Separately:
69102: gLOCATOR™ Luminescent Labels
69137: Development Folder

Related Literature:

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Material Safety Data Sheets:
69264: AP Detection Reagent Kit - English
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Related Categories:
All Categories » Calbiochem » Antibodies » Support Products » Substrates for AP, HRP, bGal and Gus
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Detection » S•Tag™ Western Blot and LumiBlot™ Kits
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Detection » AP & HRP Blot Development Substrates

Selected Citations:
  1. Alla Korepanova, et al. (2005) Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli. Protein Science 14, 148-158.