High-efficiency one-tube l packaging The Novagen PhageMaker System is optimized for maximum packaging efficiencies of cDNA and genomic DNA inserts ligated with any bacteriophage l vector. This one-tube system is extremely easy to use: simply add up to 5 µg of ligated vector plus insert to the extract and incubate at room temperature for 2 hours. Features and Benefits:
- Packaging efficiency of > 2 × 108 pfu/µg DNA
- The negligible background of < 10 pfu per extract is orders of magnitude lower than conventional two-extract systems.
- The extract is devoid of EcoK and other restriction systems (mcrA, mcrBC, mrr) that recognize methylated DNA. Therefore, the PhageMaker® system is especially recommended for constructing highly representative cDNA and genomic DNA libraries in which the insert DNA is methylated.
- The one-tube configuration enables the use of multiple aliquots from the same tube, thus providing a significant cost savings over other systems.
References:
Gunther, E.J., et al. 1983. Nucleic Acids Res. 21, 3903. Rosenberg, S.M., et al. 1985. Gene 38, 165. Rosenberg, S.M. 1987. Methods Enzymol. 153, 95. Components:
| • | 11 × 50 µl | PhageMaker Extracts
| | • | 2 µg | PhageMaker Control DNA
| | • | 0.2 ml | LE392 Host Strain |
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