 |
 | 15 to 30°C |  | Ambient or blue ice | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| |  | Cat. No. 69673 |
|
|
| Purification of His•Tag fusion proteins by metal chelation chromatography | | | The His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins. After unbound proteins are washed away, the target protein is recovered by elution with either imidazole or slight reduction in pH. This versatile system enables proteins to be purified under gentle, non-denaturing conditions, or in the presence of either 6 M guanidine or urea. Further details can be found in the His•Bind Purification Kits Overview.
*manufactured by QIAGEN | | | | | |
The Chromatography Columns are disposable polypropylene mini-columns with integral buffer reservoir. Chromatography Columns hold a bed volume of up to 5 ml and are convenient for small scale gravity flow column purification protocols. |
|
| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
|
|
