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T7Select1-2c Sequence
TB178 T7Select System Manual
TB182 T7Select 1-2a-c Vector Map
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  T7Select® 1-2c DNA
Cat. No. 70044  
All Categories » Novagen » T7Select® Phage Display & Libraries » T7Select® Vector DNA
T7Select® Vector DNA
Highly purified uncut DNA
 
T7Select® vectors are available as uncut DNA to provide maximum flexibility in cloning options. They are supplied as highly purified linear phage DNA. Three basic types of T7Select phage display vectors are available: the T7Select415-1 vector, for high-copy number display of peptides; the T7Select10- 3 vector, for mid-copy number display of peptides and proteins; and the T7Select1-1 and -2 vectors, for low-copy number display of peptides or larger proteins. In all of the vectors, sequences for the peptides or proteins to be displayed are inserted within a series of multiple cloning sites following amino acid 348 of the major capsid protein. 
 
Product Description

The T7Select1-2a-c vectors are protein display vectors based on bacteriophage T7. The vector displays an average of 0.1–1 copies of peptides and proteins up to 900 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select1 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 0.1–1 copy of 10B fusion protein per virion. The linear genome of T7Select1-2a is 37,245 bp in size. Unique restriction sites are shown on the vector map (TB182).  

Need additional information about this product? Email our Technical Service department at: novatech@novagen.com

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EMD Chemicals Inc. EUR list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

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70044-310 µgY€253


EMD Chemicals Inc. EUR list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Product Name
Cat. No.
Size
In Stock
Qty
Select Country Above
For Pricing Information
T7Select® 415-1 DNA70040-310 µgY€253
T7Select® 1-2a DNA70042-310 µgY€253
T7Select® 1-2b DNA70043-310 µg*€253
T7Select® 1-1 DNA70048-310 µgY€253
T7Select™10-3 DNA70548-310 µgY€253

* Additional Product Information
Cat. No.Disclaimers
70043-3
Stock StatusPlease contact our Customer Service Department for delivery time.

Material Safety Data Sheets:
70044: T7Select® 1-2c DNA - English
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Related Categories:
All Categories » Novagen » T7Select® Phage Display & Libraries » T7Select® Vector DNA
All Categories » Novagen » Vectors » Phage » T7Select

Selected Citations:
  1. Stefan Danner and Joel G. Belasco. (2001) T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Procedings of the National Academy of Science 98, 12954-12959.