| |  | Cat. No. 70048 |
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| Highly purified uncut DNA | | | | T7Select® vectors are available as uncut DNA to provide maximum flexibility in cloning options. They are supplied as highly purified linear phage DNA. Three basic types of T7Select phage display vectors are available: the T7Select415-1 vector, for high-copy number display of peptides; the T7Select10- 3 vector, for mid-copy number display of peptides and proteins; and the T7Select1-1 and -2 vectors, for low-copy number display of peptides or larger proteins. In all of the vectors, sequences for the peptides or proteins to be displayed are inserted within a series of multiple cloning sites following amino acid 348 of the major capsid protein. | | | | | |
The T7Select1-1b vector is a protein display vector based on bacteriophage T7. The vector displays an average of 0.1–1 copies of peptides and proteins up to 1200 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select1 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 0.1–1 copy of 10B fusion protein per virion. The linear genome of T7Select1-1b is 36,219 bp in size. Unique restriction sites are shown on the vector map (TB181). |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
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