 |
 | -20°C |  | Blue or dry ice | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| |  | Cat. No. 70049 |
|
|
| Baculovirus transfer plasmids for convenient cloning and expression | | | | The pBAC™ transfer plasmids are designed for convenient cloning and expression of target genes from baculovirus vectors. Optional features include fusion tags for detection and purification, gus marker gene for positive identification of recombinants, and co-expression of multiple genes. All pBAC transfer plasmids are supplied as highly purified supercoiled DNA. | | | | | |
pBACgus-2cp is a baculovirus transfer plasmid designed for simplified cloning and expression of target genes in insect cells. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter. The plasmid also carries the gus gene encoding b-glucuronidase under control of the late basic protein promoter (P6.9), which serves as a reporter to verify recombinant viruses by staining with X-gluc. pBACgus-2cp provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. A Ligation-Independent Cloning (LIC) version of the vector is available for rapid, directional cloning of PCR products adjacent to the enterokinase cleavage site. The vector provides optional expression of a C-terminal His•Tag fusion sequence by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map. |
|
| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| - Carla V. Finkielstein, Michael Overduin and Daniel G. Capelluto. (2006) Cell migration and signaling specificity is determined by Rac1's phosphatidylserine recognition motif. Journal of Biological Chemistry 281, 27317-27326.
| |
|
|
