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| |  | Cat. No. 70088 |
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| Baculovirus transfer plasmids for convenient cloning and expression | | | | The pBAC™ transfer plasmids are designed for convenient cloning and expression of target genes from baculovirus vectors. Optional features include fusion tags for detection and purification, gus marker gene for positive identification of recombinants, and co-expression of multiple genes. All pBAC transfer plasmids are supplied as highly purified supercoiled DNA. | | | | | |
pBAC™-3 is a baculovirus transfer plasmid compatible with BacVector™-1000, -2000, or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the polh promoter and expression of secreted proteins. A 20 amino acid gp64 signal peptide sequence is capable of directing high levels of protein into the secretory pathway of infected insect cells. Cloning sites are provided for the creation of N-terminal fusions of an insert with His•Tag® and/or S•Tag™ sequences. pBAC-3 provides an ATG start codon at the optimal position relative to native polyhedrin translation signals. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to the coding strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
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