| |  | Cat. No. 70548 |
|
|
| Highly purified uncut DNA | | | | T7Select® vectors are available as uncut DNA to provide maximum flexibility in cloning options. They are supplied as highly purified linear phage DNA. Three basic types of T7Select phage display vectors are available: the T7Select415-1 vector, for high-copy number display of peptides; the T7Select10- 3 vector, for mid-copy number display of peptides and proteins; and the T7Select1-1 and -2 vectors, for low-copy number display of peptides or larger proteins. In all of the vectors, sequences for the peptides or proteins to be displayed are inserted within a series of multiple cloning sites following amino acid 348 of the major capsid protein. | | | | | |
The T7Select™10-3b vector is a protein display vector based on bacteriophage T7. The vector displays an average of 5–15 copies of peptides and proteins up to 1200 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select10 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 5–15 copies of 10B fusion protein per virion. The linear genome of T7Select10-3b is 36,249 bp in size. Unique restriction sites are shown on the vector map (TB243). |
|
| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
|
| |
| - Eun-Min Cho, et al. (2007) Phage display cDNA cloning and expression analysis of hydrophobins from the entomopathogenic fungus Beauveria (Cordyceps) bassiana. Microbiology 153, 3438-3447.
- Inge Roman, et al. (2006) Hunting interactomes of a membrane protein: obtaining the largest set of VDAC-interacting protein epitopes. Molecular and Cellular Proteomics 5, 1667-1680.
- Z.J. Li, et al. (2003) Screening and identification of proteins interacting with p38 MAP kinase via T7 phage-display screening system. Di Yi Jun Yi Da Xue Xue Bao 23, 1131-1133.
- Alexi Kurakin and Dale Bresden. (2002) Target-assisted iterative screening reveals novel interactors for PSD95, Nedd4, Src, Ab1 and Crk proteins. Journal of Biomolecular Structure and Dynamics 19,.
- Kimmo Porkka, et al. (2002) A fragment of the HMGN2 protein homes to the nuclei of tumor cells and tumor endothelial cells in vivo. Procedings of the National Academy of Science 99, 7444-7449.
- M.P. Burgoon, et al. (2001) Antigen discovery in chronic human inflammatory central nervous disease: panning phage-display antigen libraries identifies the targets of central nervous system-derived IgG in subacute sclerosing panencephalitis. Journal of Immunology 167, 6009-6014.
- Mona H. Hansen, Bjorn Ostenstad and Mouldy Sioud. (2001) Identification of immunogenic antigens using a phage-displayed cDNA library from an invasive ductal breast carcinoma tumor. International Journal of Oncology 19, 1303-1309.
- Paul P. Sche, et al. (1999) Display cloning: functional identification of natural product receptors using cDNA-phage dispaly. Chemistry and Biology 6, 707-716.
| |
 |
 |
|
