| | BugBuster® Protein Extraction Reagent |
|  | Cat. No. 70584 |
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| Simple extraction of active protein from E. coli without sonication | | | BugBuster® Protein Extraction Reagent is formulated to gently disrupt the cell wall of E. coli and liberate soluble proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a detergent mix that is capable of cell wall perforation without denaturing soluble protein.
In practice, cells are harvested by centrifugation and suspended in BugBuster. The addition of rLysozyme™ Solution, which hydrolyzes N-acetylmuramide linkages in the cell wall, enhances the extraction efficiency, especially for larger proteins. Following a brief incubation, insoluble cell debris is removed by centrifugation. At this point, Benzonase® Nuclease can be added to reduce the viscosity of the extract due to liberation of chromosomal DNA. The clarified extract is ready to use and fully compatible with the affinity supports offered by Novagen, including GST•Bind™, GST•Mag™, His•Bind®, His•Mag™, S•Tag™, Strep•Tactin®, and T7•Tag® Resins, or several other chromatography matrices. Following binding to the affinity resin, excess BugBuster is easily removed by washing the column with the appropriate buffer. BugBuster is also useful for the preparation of high-purity inclusion bodies in instances where expressed proteins are insoluble. The reagent is available in a variety of configurations.
BugBuster Reagent and BugBuster Plus Benzonase® Nuclease
The standard BugBuster reagent is supplied as a Tris-buffered "1X" ready-to-use liquid that is stable at room temperature. The 500-ml size is also available bundled with 10 KU Benzonase Nuclease (provided in a separate vial) for the preparation of low-viscosity extracts and/or removal of nucleic acids from protein preparations. BugBuster and Benzonase are compatible with common protease inhibitors.
BugBuster Master Mix
BugBuster® Master Mix combines BugBuster Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme™ Solution in one convenient reagent. BugBuster Master Mix allows for maximum recovery of active, soluble protein from both Gram-negative and Gram-positive bacteria. With the Master Mix, there is no need for dilution or separate addition steps. The two available package sizes provide sufficient reagents for protein extraction from 20 g and 100 g cell paste.
BugBuster Plus Lysonase Kit
The BugBuster Plus Lysonase™ Kit combines the activities of both reagents to significantly increase protein extraction efficiency and facilitate downstream processing of protein extracts, thereby enabling maximum recovery of active soluble protein from both Gram-negative and Gram-positive bacteria. Lysonase Bioprocessing reagent is an optimized, ready-to-use blend of rLysozyme Solution and Benzonase Nuclease. Use 5 ml BugBuster and 10 µl Lysonase per gram of cell paste. Two kit sizes provide sufficient reagents for protein extraction from either 20 or 100 g cell paste.
BugBuster HT Protein Extraction Reagent
BugBuster HT combines BugBuster Protein Extraction Reagent and Benzonase Nuclease in one convenient reagent. BugBuster HT eliminates common bioprocessing problems resulting from traditional cell lysis procedures. Soluble proteins are gently extracted from E. coli without exposure to heat or oxidative damage and viscosity is eliminated by nucleic acid digestion in a single step. The resulting protein extract can easily be fractionated by conventional purification techniques. BugBuster HT is ideally suited for application in high-throughput protein purifications. Compatible with rLysozyme Solution.
BugBuster 10X Protein Extraction Reagent
BugBuster 10X is a concentrated formulation of the proprietary detergents employed in BugBuster without the addition of buffer components. Concentrated BugBuster provides a flexible alternative to the ready-to-use standard 1X BugBuster, allowing user-defined dilution and addition of buffer components. BugBuster 10X has all of the bioprocessing benefits of standard BugBuster plus the freedom to control pH, reagent concentration, and buffer additives necessary for maximum extraction and activity of your target protein.
BugBuster (primary amine-free) Protein Extraction Reagent
BugBuster (primary amine-free) is a special formulation of BugBuster designed for applications where primary amines would interfere if present in the protein extract, such as protein immobilization or cross-linking. The PIPPS buffer used in BugBuster (primary amine-free) has a similar buffer capacity and pH range as the original Tris-buffered BugBuster, but will not complex metal ions, also making it ideally suited for extraction of metal-dependent proteins. | | | | |
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| BugBuster® plus Benzonase® Nuclease | 70750-3 | 1 kit | Y | N/A |
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