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| High molecular weight, high purity DNA for any application | | | | Genomic DNAs are highly purified preparations of high molecular weight genomic DNA from a variety of organisms. The isolation protocol minimizes shearing and removes contaminants that inhibit enzymatic procedures. Novagen genomic DNA is qualified for genomic analysis (including PCR) and library construction. At least 90% of the DNA is greater than 100 kbp in size, as analyzed by CHEF gel electrophoresis (> 50 kbp for S. cerevisiae). No inhibition of restriction enzyme activity is observed, and the DNA is free of contaminating RNA and protein. The DNA is supplied in 10 mM Tris-HCl, pH 8.0, 1 mM EDTA (TE). Pipet with a wide-bore pipet tip to minimize shearing. | | | 
Size analysis
CHEF analysis of duplicate samples run in 1.2% agarose and stained with ethidium bromide. Conditions were 180 V, 18 h, 2 s initial switch interval ramped to 10 s, 0.5X TBE, 25°C.
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