Non-fluorescent visible DNA co-precipitant for automated sequencing applications 
Pellet Paint NF Co-Precipitant is a nonfluorescent dye-labeled carrier compatible with fluorescent sequencing. It facilitates rapid removal of BigDye Terminators during the alcohol precipitation of cycle sequencing reaction products. Cycle sequencing reactions can be precipitated rapidly with 1 µl of carrier per reaction and centrifugation times of 10 minutes. The easily visualized carrier provides a simple confirmation that precipitation has occurred. Sequencing reaction products are efficiently pelleted and dye-labeled terminators remain in the supernatant during alcohol precipitation using the standard Applied Biosystems precipitation protocols. Resuspension of pelleted sequencing reaction products in deionized formamide can be confirmed by checking for dissolution of the carrier. Pellet Paint NF Co-Precipitant is fully compatible with the ABI PRISM® BigDye Terminator Cycle Sequencing Ready Reaction. To avoid extra sample handling, Pellet Paint NF Co-Precipitant can be added directly to the reaction mix, template DNA, crude PCR samples, or dilution buffer before the cycle sequencing reaction. Although Pellet Paint NF absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet Paint NF) can be used as a correction factor when determining nucleic acid concentration. Pellet Paint NF Co-Precipitant has no detectable effect on the sequencing reaction or sequence accuracy. Pellet Paint NF Co-Precipitant is a useful substitute for the original Pellet Paint Co-Precipitant in applications where fluorescent detection is used.
Features and Benefits:
- Efficient and rapid precipitation of BigDye® cycle sequencing products
- Efficient removal of dye terminators
- Direct visualization and tracking of precipitated material
- No effect on sequencing reaction
- Substitute for original Pellet Paint® Co-Precipitant for fluorescent detection applications
Note: European Patent No. EP0853680 has been issued to EMD Biosciences, Inc./Novagen for a method for precipitating nucleic acid with a visible carrier. US Patent pending Components:
| • | 125 µl or 1 ml | Pellet Paint NF Co-Precipitant
| | • | 1 ml or 8 ml | 3 M Sodium Acetate, pH 5.2 |
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