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 | 15 to 30°C |  | Ambient or blue ice |  | Multiple Toxicity Values, refer to MSDS | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. 70899 |
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| Purification of His•Tag fusion proteins by metal chelation chromatography | | | The His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins. After unbound proteins are washed away, the target protein is recovered by elution with either imidazole or slight reduction in pH. This versatile system enables proteins to be purified under gentle, non-denaturing conditions, or in the presence of either 6 M guanidine or urea. Further details can be found in the His•Bind Purification Kits Overview.
*manufactured by QIAGEN | | | | | |
The Ni-NTA Buffer Kit provides a convenient set of buffers optimized for purification of His•Tag fusion proteins on Ni-NTA His•Bind Resin. These phosphate-buffered solutions differ from the Tris-based solutions used in the His•Bind Buffer Kit. Carefully prepared 4X concentrates are included for binding, washing and elution according to recommended protocols. Components:
| • | 2 × 125 ml | 4X Ni-NTA Bind Buffer
| | • | 125 ml | 4X Ni-NTA Wash Buffer
| | • | 50 ml | 4X Ni-NTA Elute Buffer |
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
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| - Torsten Kessler, et al. (2005) Inhibition of tumor growth by RGD peptide-directed delivery of truncated tissue factor to the tumor vasculature. Clinical Cancer Research 11, 6317-6324.
- Ying Liu, Zhi-Xing Yao and Vassilios Papadopoulos. (2005) Cytochrome p450 17α hydroxylase/17,20 lyase (CYP17) function in cholesterol biosynthesis: identification of squalene monooxygenase (epoxidase) activity associated with CYP17 in Leydig cells. Molecular Endocrinology 19, 1918-1931.
- Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189–194.
- Aileen Aherne, et al. (2004) On the molecular pathology of neurodegeneration in IMPDH1-based retinitis pigmentosa. Human Molecular Genetics 13, 641-650.
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