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 | 2 to 8°C |  | Blue ice |  | Hazardous |  | Flammable | | Note: Store and Ship conditions may differ. | | See Key |
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| | GeneJuice® Transfection Reagent |
|  | Cat. No. 70967 |
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High-efficiency transfection of mammalian cells GeneJuice® Transfection Reagent is a proprietary formulation optimized for maximal transfection efficiency, ease of use, and minimal cytotoxicity. This transfection reagent is a superior alternative to a wide variety of other techniques including calcium phosphate coprecipitation, electroporation, microinjection, biolistic particle delivery, and complex formation with DEAE-dextran.
GeneJuice Transfection Reagent provides excellent performance in both stable and transient transfection of eukaryotic cells and is ideal for use with the Novagen pTriEx™ expression vectors.
The 1-ml size provides enough reagent to perform up to 500 transfections in standard 35-mm plates. The reagent is also available in an introductory 0.3-ml size, a convenient, new 5 × 1-ml size, and a 10-ml size. GeneJuice Transfection Reagent is supplied as a ready-to- use sterile solution.
www.novagen.com/transfection
Features and Benefits:
- Highly efficient DNA transfer for both stable and transient transfections
- Minimal cellular toxicity
- Compatibility with both serum-containing and serum-free media
- Simple protocol—no need for media changes
- Ideal for high-throughput transfection in a multi-well plate format
Learn more about our transfection reagents.
Cell lines transfected with GeneJuice™ Transfection Reagent
| Cell Lines: | Primary Cells: |
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10T1/2
293T
3T3 NIH
3T3 Swiss
3T3-L1
A204
A431
A549
alpha TC1-6
AR 42J
As4.1
AtT-20
B50
BC-1
BC-2
BC3
BCBL
BHK-21
C3H/10T1/2
C6
C2C12 | Caco-2
Caki-1
Calpan-1
Calu-1
Calu-6
CCL-131
CFPAC-1
Chang Liver
CHO
CHO-7
CHO-IR
CHO-K1
COS-1
COS-7
CS-1
CV-1
Daudi
DDTI MF-2
DT40
ECV304
EL4 | ES-E14TG2a
EVSCC17M
H9c2
HCT-116
HEK293
HeLa
HeLa B
HeLa T4
Hep 3B2.1-7
HepG2
Hepa 1-6
Ht-29
HTB-37
HTB-45
Huh-7
HUVEC
IC21
IEC-6
JEG-3
Jurkat
KB | L57-3-11
L-6
L-929
MA-10
McA-RH7777
MCF-7
MCF-10-2A
MDCK
Melanocyte
MG-63
Neuro 2A
Neuroblastoma
NPK
NT2/D1
OV-1063
OVCAR3
P4
P19
PC12
PA317
PAM212 | PS-1
R2C
RAW 264.7
RBL-2H3
RMP-41
SAOS-2
SC-1
Schneider line2
SK-N-MC
SK-N-SH
SKOV3
STO
SW-480
SW-837
T3M4
TM4
U937
UCD
Vero
WE-38
| Aortic smooth muscle cells
Astrocytes
Angioblasts
Chondrocytes
Chromaffin cells
Epithelial cells:
mammary
prostate
tracheal
Fibroblasts
Keratinocytes
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
 | Related information for this product is available: | |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
GeneJuice™ transfection of Cos-7 and HeLa cells with rhodamine-labeled DNA
Cells grown on polylysine-coated coverslips to 50% confluency were transfected with rhodamine-labeled pTriEx™-2 DNA using GeneJuice. Labeled plasmid DNA was complexed with GeneJuice in serum-free medium, and the complexes were added directly to the cells in complete medium. Twenty-four hours after transfection, the cells were washed in PBS, fixed in 4% formalin for 10 minutes, and washed again in PBS. Coverslips were mounted on glass slides and sealed for confocal microscopy. The transfected DNA is seen in red. Unfiltered reflected light from the 533 nm laser was collected to image the cell boundaries. |
| 345810: G 418 Sulfate, Cell Culture Tested
345812: G 418 Sulfate, Sterile-Filtered Aqueous Solution, Cell Culture Tested
400052: Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in PBS, Cell Culture-Tested
400053: Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested
4801: Neomycin Sulfate
480100: Neomycin Sulfate, g-Irradiated, Tissue Culture Grade
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| - Veronica Athie-Morales, Geraldine M. O'Connor and Clair M. Gardiner. (2008) Activation of human NK cells by the bacterial pathogen-associated molecular pattern muramyl dipeptide. Journal of Immunology 180, 4082-4089.
- Padraig D'Arcy, et al. (2008) The oncoprotein SS18-SSX1 promotes p53 ubiquitination and degradation by enhancing HDM2 stability. Molecular Cancer Research 6, 127-138.
- Daniel P. Morris, et al. (2008) The α1a-adrenergic receptor occupies membrane rafts with its G protein effectors but internalizes via clathrin-coated pits. Journal of Biological Chemistry 283, 2973-2985.
- Robert J. Wood and Mark D. Hulett. (2008) Cell surface-expressed cation-independent mannose 6-phosphate receptor (CD222) binds enzymatically active heparanase independently of mannose 6-phosphate to promote extracellular matrix degradation. Journal of Biological Chemistry 283, 4165-4176.
- Makiko Yokouchi, et al. (2008) Involvement of selective reactive oxygen species upstream of proapoptotic branches of unfolded protein response. Journal of Biological Chemistry 283, 4252-4260.
- Niamh E. Buckley, et al. (2007) BRCA1 regulates IFN-g signaling through a mechanism involving the type I IFNs. Molecular Cancer Research 5, 261-270.
- Rossitza Christova, et al. (2007) P-STAT1 mediates higher-order chromatin remodelling of the human MHC in response to IFN. Journal of Cell Science 120, 3262-3270.
- Sebastian Herzog and Hassan Jumaa. (2007) The N Terminus of the Non-T Cell Activation Linker (NTAL) Confers Inhibitory Effects on Pre-B Cell Differentiation. Journal of immunology 178, 2336-2343.
- Leo T. O. Lee, et al. (2007) Discovery of growth hormone-releasing hormones and receptors in nonmammalian vertebrates. Procedings of the National Academy of Science 104, 2133-2138.
- Jeong Yong Lee, et al. (2007) Induction of glutathione transferase in insulin-like growth factor type I receptor-overexpressed hepatoma cells. Molecular Pharmacology 72, 1082-1093.
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