Rapid purification of PCR products for downstream procedures The SpinPrep™ PCR Clean-Up Kit is designed for the rapid purification of DNA amplified in PCR. The 10-minute procedure involves addition of a binding buffer followed by adsorption of the DNA to a silica membrane in a spin column format. Following a wash step, the DNA is eluted in low-salt buffer. This kit removes DNA polymerases, dNTPs, salts, and > 99% of primers so that they do not interfere with downstream applications such as cloning, sequencing, or labeling. PCR products from 100 bp to > 12,000 bp can be cleaned up, with recoveries of amplified DNA in the range of 60-90% under standard conditions.
| | | | Column binding capacity: | up to 6 µg | | PCR volume: | 100 µl/rxn | | Typical recovery: | 60–90% | | Size range: | 100 bp to > 12,000 bp | | Time required: | < 10 min |
 Components:
| • | 82 ml | SpinPrep Bind Buffer
| | • | 27 ml | SpinPrep Wash Buffer
| | • | 10 ml | SpinPrep Elute Buffer
| | • | 100 | SpinPrep Filters
| | • | 100 | Receiver Tubes
| | • | 100 | Eluate Receiver Tubes |
|
