High performance enzyme blend for long and accurate PCR KOD XL DNA Polymerase* is an optimized blend of KOD DNA Polymerase and a mutant form of KOD that is deficient in 3′→5′ exonuclease activity (Nishioka 2002). This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of derivatized dNTPs in PCR amplicons (Sawai 2002, Sawai 2001). KOD XL DNA Polymerase generates a mixture of PCR products with blunt and 3′-dA overhangs, suitable for cloning with Novagen Perfectly Blunt®, AccepTor™, and LIC Vector Kits.
 Features and Benefits:
- Ideal for amplification of large DNA fragments from purified DNA or crude samples
- Amplifies DNA templates up to 30 kbp
- Successfully amplifies GC-rich sequences
- Efficiently incorporates derivatized dNTPs
References:
Nishioka, M., et al. 2002. J. Biotechnol. 88, 141. Sawai, H., et al. 2002. Bioconjugate Chem. 13, 309. Sawai, H., et al. 2001. Chem. Commun. 24, 2604. | Source | Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli (wild type and exonuclease-deficient forms) | | Concentration | 2.5 U/µl | | Endonuclease | None detected | | Nicking activity | None detected | | Amplification effiency | Functional PCR | | Storage | –20°C |
*Manufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Licensed under US Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA. Unit Activity: One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA. Components:
| • | 250 U or 5 × 250 U | KOD XL DNA Polymerase (2.5 U/µl)
| | • | 1.2 ml or 5 × 1.2 ml | 10X PCR Buffer for KOD XL DNA Polymerase
| | • | 1 ml or 5 × 1 ml | dNTP Mix (2 mM each) |
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