PopCulture® extraction and GST•Mag purification in a 96-well plate The RoboPop™ GST•Mag™ Purification Kit is designed for 96-well format purification of GST•Tag™ fusion proteins directly from E. coli cultures without harvesting cells. The kit features PopCulture® Reagent for extraction of proteins from total cultures without the need for centrifugation, and GST•Mag Agarose Beads for high-capacity magnetic affinity purification. The combination of PopCulture and magnetic agarose beads enables the entire procedure to be carried out in a single culture plate. The kit contains one Sterile 96-well Deep Well Culture Plate (2-ml wells) with three air-permeable sealing membranes for bacterial cell growth and protein purification, and one 96-well Collection Plate (450-µl wells) with an air-tight aluminum foil sealer for storage of the purified proteins. rLysozyme™ Solution, Benzonase® Nuclease, and purification buffers are also included. The Culture Plate is compatible with the Novagen Magnetight™ HT96™ Separation Stand, which is recommended for efficient processing of magnetic affinity supports in deep-well plates. The Sterile 96-well Deep Well Culture Plate with Sealers is available separately. The RoboPop GST•Mag Purification Kit will purify up to 4.8 mg of GST•Tag fusion proteins per plate (up to 50 µg/well). Stated yields are based on 1-ml cultures and binding capacities of the beads, and will vary with the folding properties, expression levels, and solubility of individual fusion proteins. The RoboPop His•Mag Purification Kit has been validated for robotic sample processing with the Genesis® Freedom™ Workstation from Tecan and the MultiPROBE® II liquid handling work station from PerkinElmer Life Sciences.
96-well Deep Well Culture Plate
Additional Sterile 96-well Deep Well Culture Plates (2-ml wells) are available as 5 plates with 5 sealers.
 Components:
| • | 15 ml | PopCulture® Reagent
| | • | 1 | Sterile 96-well Deep Well Culture Plate
with Sealers (3)
| | • | 1 | Collection Plate with Sealer
| | • | 3 x 1 ml | GST•Mag Agarose Beads
| | • | 2 x 100 ml | 10X GST Bind/Wash Buffer
| | • | 40 ml | 10X GLutathione Reconstitution Buffer
| | • | 1 g | Reduced Glutathione
| | • | 300 KU | rLysozyme™ Solution
| | • | 1 ml | rLysozyme Dilution Buffer
| | • | 2.5 KU | Benzonase® Nuclease,
Purity > 90% |
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