PopCulture® extraction and His•Mag™ or GST•Mag™ purification in a 96-well format The RoboPop™ Purification Kits are designed for 96-well format purification of soluble His•Tag® fusion proteins directly from E. coli cultures without harvesting cells. The kits feature PopCulture® Reagent for extraction of proteins from total cultures without the need for centrifugation, and His•Mag™ Agarose Beads for high-capacity magnetic affinity purification. The combination of PopCulture and magnetic agarose beads enables the entire procedure to be carried out in a single culture plate.
The kits contain one 96-well Deep Well Culture Plate (2-ml wells) with three airpermeable sealing membranes for bacterial cell growth and protein purification, and one 96-well Collection Plate (450-µl wells) with an air-tight aluminum foil sealer for storage of the purified proteins. rLysozyme™ Solution, Benzonase® Nuclease, and purification buffers are also included.
The Culture Plate is compatible with the Novagen Magnetight™ HT96™ Separation Stand, which is recommended for efficient processing of magnetic affinity supports in deep well plates.
The RoboPop His•Mag Purification Kits will purify up to 12 mg of His•Tag fusion proteins per plate (up to 125 µg/well). Stated yields are based on 1-ml cultures and binding capacities of the beads, and will vary with the folding properties, expression levels, and solubility of individual fusion proteins. The kit has been validated for robotic same processing with the Genesis® Freedom™ Workstation from Tecan and the MultiPROBE® II workstation from PerkinElmer Life Sciences.
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 Components:
| • | 15 ml | PopCulture® Reagent
| | • | 1 | Sterile 96-well Deep Well Culture Plate
with Sealers (3)
| | • | 1 | Collection Plate with Sealer
| | • | 3 x 1 ml | His•Mag Agarose Beads
| | • | 80 ml | 8X Binding Buffer
| | • | 2 x 25 ml | 8X Wash Buffer
| | • | 50 ml | 4X Elute Buffer
| | • | 300 KU | rLysozyme™ Solution
| | • | 1 ml | rLysozyme Dilution Buffer
| | • | 2.5 KU | Benzonase® Nuclease,
Purity > 90% |
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