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 | -20°C |  | Blue ice | | Note: Store and Ship conditions may differ. | | See Key |
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| |  | Cat. No. 71110 |
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Stabilized recombinant lysozyme rLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3-5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45-60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration of 30 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at -20°C.
 Unit Activity: One unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A450 unit per minute at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5. |
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EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
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| - Rachel Balder, et al. (2007) Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Infection and Immunity 75, 2765-2775.
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- B. Husen, et al. (2003) Characterization of 17β-hydroxysteroid dehygronase type 7 in reproductive tissues of the marmoset monkey. Biology of Reproduction 68, 2092-2099.
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