Immobilized antibody in 96-well format The His•Tag® Antibody Plate is a 96-well, ELISA-compatible plate containing immobilized His•Tag Monoclonal Antibody. The antibody is covalently immobilized to the surface using a method that retains maximal binding activity. The antibody specifically recognizes five consecutive histidines, and so will bind with high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) to virtually any His•Tag fusion protein in which the tag is exposed. This plate has outstanding binding characteristics, with a capacity of > 100 ng His•Tag fusion protein per well and low non-specific binding. Well-to-well variability is less than 5% and the plates are stable when stored dry at 4°C. The His•Tag Antibody Plate can be used in a variety of binding assays where reliable, specific immobilization of His•Tag fusion proteins is required.
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