Protein extraction directly from insect cell cultures

Insect PopCulture^® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind^® affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.

Features and Benefits:

• No need to separate cells from culture media
• No need to clarify cell extracts prior to purification
• Higher protein yield due to target protein recovery from both medium and cells
• Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
• Compatible with protease inhibitor cocktails
• Ideal for high-throughput, expression-level screening and protein purification
• Compatible with transfected and baculovirus-infected insect cell cultures

References:
Loomis, K., et al. 2002. inNovations 15, 16.