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| |  | Cat. No. 71233 |
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| Nonlytic expression in insect cells plus tightly regulated E. coli expression | | | The pBiEx™ Vectors are designed to allow rapid characterization of target genes in both E. coli and insect cells, and to allow high-level transient protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. The vectors feature the same hr5 enhancer and the ie1 (immediate early) promoter to direct expression in insect cells as the pIEx™ Vectors, and also allow for expression in E. coli by the tightly controlled T7lac promoter. For target protein expression, pBiEx recombinant plasmids can be transferred into a DE3 host that allows induction with IPTG. Expression can also be induced in hosts such as NovaBlue by infecting with lCE6, a phage that constitutively expresses T7 RNA polymerase. Three vectors offer a variety of N-terminal and optional C-terminal fusion tags as well as choices for no fusion tags.
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InsectDirect™ System- pBiEx™-2 DNA The pBiEx™ vectors are designed for cloning and high-level expression of proteins in both bacteria and in transiently transfected Spodoptera-derived insect cells. In E. coli, target gene transcription is regulated by the tightly controlled T7lac promoter, and a ribosome binding site (rbs) directs translation initiation. In insect cells, transcription is driven by the AcNPV-derived hr5 enhancer and IE1 immediate early promoter.
pBiEx-2 contains N-terminal GST•Tag, His•Tag and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. For sequencing or PCR of recombinants in pBiEx-2, refer to the table below to choose the appropriate primers.
This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. |
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| Need additional information about this product? Email our Technical Service department at: novatech@novagen.com |
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