Return to our Home Page
Novagen Home Page

Tech Resources
TB350 pIEx™-4 Vector Map
TB356 InsectDirect™ pIEx™ Vectors
pIEx™-4 Vector Sequence
MSDS - English
Other Languages
Store
-20°C-20°C
Ship
Shipped with Blue Ice or with Dry IceBlue or dry ice
Note: Store and Ship conditions may differ.
See Key

Dual Use Insect-Baculovirus Vectors
  pIEx™-4 DNA
Cat. No. 71235  
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Insect Cell Expression » pIEx™ Insect Cell Expression Plasmids
pIEx™ Insect Cell Expression Plasmids
High-yield, nonlytic expression in insect cells
 
The pIEx™ vectors are designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. 
 
Product Description

InsectDirect™ System- pIEx™-4 DNA

The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1.

pIEx-4 contains C-terminal S•Tag and His•Tag coding sequences. For sequencing or PCR of recombinants in pIEx-4, refer to the table below to choose the appropriate primers.

Vector5′ Primer3′ Primeralt 5′ Primer
pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
NA
pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
NA
pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
T7 Promoter Primer
pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
T7 Promoter Primer
pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer
 

Need additional information about this product? Email our Technical Service department at: novatech@novagen.com

 Related information for this product is available:

EMD Chemicals Inc. list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.

Cat. No.
Size
In Stock
Select Country Above
For Pricing Information
71235-320 µgYN/A

Related Literature:

InsectDirect™ Protein Expression & Purification System

Insect Cell Expression

Material Safety Data Sheets:
71235: pIEx™-4 DNA - English
Bulgarian
Danish
Dutch
Finnish
French
German
Greek
Hungarian
Italian
Korean
Norwegian
Polish
Portuguese
Spanish
Swedish

Related Categories:
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Insect Cell Expression » pIEx™ Insect Cell Expression Plasmids
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Insect Cell Expression » InsectDirect™ System
All Categories » Novagen » Protein Expression, Purification, and Detection » Protein Expression » Insect Cell Expression » pIEx™ and pBiEx™ Expression Vectors
All Categories » Novagen » Vectors » Plasmid » pIEx