InsectDirect™ System- pIEx™-3 DNA

The pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-3 contains the coding sequence for the signal peptide of adipokinetic hormone (AKH) to allow secretion of the expressed protein. pIEx-3 also contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. Unique sites are shown on the circle map. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
pIEx-3 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

For sequencing or PCR of recombinants in pIEx-3, refer to the table below to choose the appropriate primers.

Vector	5' Primer	3' Primer	alt 5' Primer
pIEx-1	S•Tag 18mer Primer	AS HSV•Tag Primer	IE1 Promoter Primer
pIEx-2	S•Tag 18mer Primer	AS HSV•Tag Primer	IE1 Promoter Primer
pIEx-3	S•Tag 18mer Primer	AS HSV•Tag Primer	IE1 Promoter Primer
pIEx-4	IE1 Promoter Primer	AS S•Tag 18mer Primer	NA
pIEx-5	IE1 Promoter Primer	AS S•Tag 18mer Primer	NA
pBiEx-1	S•Tag 18mer Primer	AS HSV•Tag Primer	IE1 Promoter Primer T7 Promoter Primer
pBiEx-2	S•Tag 18mer Primer	AS HSV•Tag Primer	IE1 Promoter Primer T7 Promoter Primer
pBiEx-3	IE1 Promoter Primer	AS S•Tag 18mer Primer	T7 Promoter Primer