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 | ≤ -70°C |  | Dry ice |  | Toxic | | Note: Store and Ship conditions may differ. | | See Key |
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| | PhosphoSafe™ Extraction Reagent |
|  | Cat. No. 71296 |
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When phosphorylation state matters PhosphoSafe™ Extraction Buffer efficiently extracts cytosolic proteins from mammalian and insect cells while preserving their phosphorylation state. This reagent contains the same formula as CytoBuster™ Protein Extraction Reagent (Trim 2001), but also includes four phosphatase inhibitors: sodium fluoride, sodium vanadate, b-glycerophosphate, and sodium pyrophosphate. PhosphoSafe Extraction Reagent is compatible with kinase assays, protein interaction analysis, and other applications. This reagent is provided in convenient 25-ml bottles (1 bottle for 71296-3, 5 bottles for 71296-4). Features and Benefits:
- Phosphorylation state preserved during extraction
- Compatible with kinase assays
References:
Trim, J.E. and Sawyer, D.L. 2001. inNovations 12, 5.
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
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| - Yanxia Liu, et al. (2007) A genomic screen for activators of the antioxidant response element. Procedings of the National Academy of Science 104, 5205-5210.
- Elena Martinelli, et al. (2007) HIV-1 gp120 inhibits TLR9-mediated activation and IFN-α secretion in plasmacytoid dendritic cells. Procedings of the National Academy of Science 104, 3396-3401.
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- Marisol Quintero, et al. (2006) Mitochondria as signaling organelles in the vascular endothelium. Proceedings of the National Academy of Sciences (USA) 103, 5379-5384.
- Phuong Thi Nguyen Sarkis, et al. (2006) STAT1-independent cell type-specific regulation of antiviral APOBEC3G by IFN-α. Journal of Immunology 177, 4530-4540.
- Anthony R. White, et al. (2006) Degradation of the alzheimer disease amyloid -peptide by metal-dependent up-regulation of metalloprotease activity. journal of Biological Chemistry 281, 17670-17680.
- Ralf Gutzmer, et al. (2005) Histamine H4 receptor stimulation suppresses IL-12p70 production and mediates chemotaxis in human monocyte-derived dendritic cells. Journal of Immunology 5224-5232.
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