The pRSF-2 Ek/LIC Vector is a LIC-compatible derivative of pRSF-1b. The plasmid contains a strong T7lac promoter and an amino-terminal His•Tag^® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional S•Tag™ coding sequence is present at the 3′ end of the MCS for generating a carboxy-terminal tag compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet Adaptors for coexpression of two proteins as well as with pET-46 and pCDF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.

References:
1. Kim, J.S. and Raines, R.T. (1993) Protein Science 2, 348–356.

Components:

•	1 µg	Ek/LIC Vector
•	25 units	T4 DNA Polymerase, LIC-qualified
•	50 µl	25 mM EDTA
•	40 µl	25 mM dATP
•	8 µl	Ek/LIC b-gal Control Insert
•	100 µl	100 mM DTT
•	50 µl	10X T4 DNA Polymerase Buffer
•	1.5 ml	Nuclease-free Water
•	5 × 2 ml	SOC Medium
•	10 µl	Test Plasmid
•	0.2 ml	BL21(DE3)pLysS Competent Cells
•	0.2 ml	BL21(DE3) Competent Cells
•	22 × 50 µl	NovaBlue GigaSingles™ Competent Cells