The Gateway® Nova pET-53-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal His•Tag® coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal Strep•Tag® II coding sequence. The presence of "gentle elution" tags at both the N- and C-termini is ideal for dual purification strategies designed to isolate full-length fusion proteins. The expression kit contains Nova F- Competent Cells for cloning, RosettaTM 2 (DE3) Competent Cells for expression, control plasmids for transformation and recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.