The Gateway® Nova pET-54-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal His•Tag® coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target in the recombinant pEXPR clone will also be fused to a C-terminal S•Tag™ coding sequence. The S•Tag sequence allows quantification of the fusion protein using the FRETWorks™ assay (Cat. No. 70724). The S•Tag can also be used for purification and detection of the fusion protein. The expression kit contains Nova F- Competent Cells for cloning, RosettaTM 2 (DE3) Competent Cells for expression, control plasmids for transformation and recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.