The Gateway® Nova pET-56-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal S•Tag™ coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target in the recombinant pEXPR clone will also be fused to a C-terminal His•Tag® coding sequence. The S•Tag sequence allows quantification of the fusion protein using the FRETWorks™ assay (Cat. No. 70724). The tag can also be used for purification and detection. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.