The Gateway® Nova pET-57-DEST™ vector is designed to create expression clones (pEXPR) that are fused to N-terminal His•Tag® and Nus•Tag™ coding sequences and a thrombin cleavage site. The Nus•Tag fusion is included to enhance the solubility of the fusion protein. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target in the recombinant pEXPR clone will also be fused to a C-terminal Strep•Tag® II coding sequence. The presence of a "gentle elution" tag at each terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.