The Gateway® Nova pET-60-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal GST•Tag™ coding sequence and a thrombin cleavage site. The GST•Tag enables gentle purification of fusion proteins using GST•Bind® resin. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal His•Tag® coding sequence. The presence of a "gentle elution" tag at each terminus is ideal for dual purification strategies designed to isolate full-length fusion proteins. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and for recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.