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  Suicide Track™ DNA Ladder Isolation Kit
Cat. No. AM41  
All Categories » Calbiochem » Kits and Tools » Apoptosis Activity and Detection Kits » Other

Format: Agarose gel electrophoresis
Form: 25 Extractions
Detection Method: Ethidium bromide
Species Reactivity: a broad range of species
Assay Time: 27 h
Sample Type: Adherent or suspension cells
Kit Contents: Extraction Buffer, Lysis Buffer, RNase Solution, DNA Isolation Solution, Pellet Paint® Co-Precipitant (Cat. No. 69049-3), DNA Ladder Control, Loading Buffer, DNA Markers, Resuspension Buffer, and a user protocol.
Comments: Two optimized protocols are included to allow for the visualization of either DNA ladder fragments only or both fragments and intact DNA.
Ref.: Earnshaw, W. C. 1995. Current Opinion in Cell Biology 7, 337. Miller,T.E., et al. 1994. In Apoptosis II: The Molecular Basis of Apoptosis in Disease, Current Communications in Cellular and Molecular Biology 8, 357. Miller, T.E., 1993. BioTechniques 15, 1042. Schwartzman, R. A., and Cidlowski, J. A. 1993. Endocrine Reviews 14, 133. Fawthrop, D.J., et al. 1991. Arch. Toxicol. 65, 437. Wyllie, A.H. 1980. Nature 284, 555. Wyllie A.H., et al. 1980. Int. Rev. Cytol. 68, 251. Kerr, J.F.R., et al. 1972. Br. J. Cancer 26, 239.
R: 20-36/37/38-41-42/43; S: 26-36

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Gel. Agarose gel electrophoresis of DNA isolated from promyelocytic leukemia cells (HL-60) using Cat. No. AM41. HL-60 cells were either untreated (Lane 1) or treated with 0.5 mg/ml actinomycin D (Lane 2). DNA Markers (M) were electrophoresed as a base pair reference.

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AM41: Suicide Track™ DNA Ladder Isolation Kit - English
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