 |
 | -20°C |  | Blue ice | | Note: Store and Ship conditions may differ. | | See Key |
|
|
|
|
| | Cleaved Caspase Antibody Sampler Kit |
|  | Cat. No. AP1026 |
|
| Host: Rabbit or Mouse | | Isotype: IgG | | Immunogen: a series of synthetic peptides corresponding to N-terminal amino acids adjacent to the proteolytic cleavage sites of human caspase-3, -7, -9, and rat caspase-6, and amino acids adjacent to the cleavage site of human PARP | | Form: Liquid | | Formulation: Caspase and PARP antibodies are supplied as 40 µl in 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. Goat anti-rabbit IgG HRP conjugate is supplied as 100 µl in 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA 50% glycerol, pH 7.5. | | Preservative: None | | Kit Contents: 40 ml each of antibodies specific for following cleaved caspases: caspase-3 (Asp175), caspase-6 (Asp162), caspase-7 (Asp198), caspase-9 (Asp315), caspase-9 (Asp330), PARP (Asp214), and 100 ml anti-rabbit IgG, peroxidase conjugate for detection. | | Comments: A convenient set of affinity purified antibodies useful for the detection of activated caspases. | | Ref.: Budihardjo, I., et al. 1999. Annu. Rev. Cell Dev. Biol. 15, 269. Nakagawa, T., et al. 1999. Nature 403, 98. Baker, S.J., and Reddy, E.P. 1998. Oncogene 17, 3261. |
|
 | Related information for this product is available: | |
| |
EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
Detection of cleaved caspase-3 by immunoblotting. Samples: Cell lysates from HeLa, NIH/3T3, and C6 cells, untreated, staurosporine-treated (1 mM) or cytochrome c-treated (0.25 mg/ml). Primary antibody: Anti-Cleaved Caspase-3 (Asp175) (Cat. No. AP1027) (1:1000). Detection: chemiluminescence.
Detection of cleaved caspase-6 by immunoblotting. Samples: Cell lysates from Jurkat cells, untreated (-) or etoposide-treated (+), and NIH/3T3 and C6 cells, untreated or staurosporine-treated. Primary antibody: Anti-Cleaved Caspase-6 (Asp162) (1:1000). Detection: chemiluminescence.
Detection of cleaved caspase-7 by immunoblotting. Samples: Cell lysates from Jurkat and HEK293 cells, untreated, cytochrome c-treated (0.25 mg/ml, 1 hour), or etoposide-treated (25 mM, 5 hours). Primary antibody: Anti-Cleaved Caspase-7 (Asp198) (1:1000). Detection: chemiluminescence.
Detection of cleaved human caspase-9 (Asp315) by immunoblotting (top panel). Samples: Cell lysates from staurosporine-treated (1 mM) HeLa cells and cytochrome c-treated Jurkat cells. Primary antibody: Anti-Cleaved Caspase-9 (Asp315) (Cat. No. AP1028) (1:1000). Detection: chemiluminescence. Detection of cleaved human caspase-9 (Asp330) by immunoblotting (bottom panel). Samples: Cell lysates from staurosporine-treated (1 mM) HeLa cells and etoposide-treated Jurkat cells. Primary antibody: Anti-Cleaved Caspase-9 (Asp330) (1:1000). Detection: chemiluminescence.
|
| | |
|
|
