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| | Anti-Cleaved Caspase-3 (Asp175) Rabbit pAb |
|  | Cat. No. AP1027 |
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| Host: Rabbit | | Isotype: IgG | | Immunogen: a synthetic peptide corresponding to amino acids adjacent to the Asp175 cleavage site of human caspase-3 | | Form: Liquid | | Formulation: In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | | Preservative: None | | Positive Control: HeLa, NIH3T3, or C6 cells treated with staurosporine (Cat. No. 569397) | | Comments: Recognizes the ~17-19 kDa large fragment of cleaved caspase-3. Does not recognize full-length caspase-3 or other cleaved caspases. | | Ref.: Decaudin, D., et al. 2002. Cancer Res. 62, 1388. Wang, J., et al. 2001. Proc. Natl. Acad. Sci. USA 98, 4038. Di Cunto, F., et al. 2000. Neuron 28, 115. Fernandes-Alnemri, T., etal. 1994. J. Biol. Chem. 269, 30761. |
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EMD Chemicals Inc. USD list price is displayed (pricing with local distributors may vary). NOTE: In Stock status is based on item availability worldwide.
| | human, mouse, rat | FC, IB, IC, PS
See Key |
Detection of human, mouse, and rat cleaved caspase-3 by immunoblotting. Samples: Whole cell lysates from untreated, staurosporine-treated (Cat. No. 569397; 1 µM), or cytochrome c-treated (250 µg/ml) HeLa, NIH 3T3, and C6 cells. Primary antibodies: Anti-Caspase-3 (upper) and Anti-Cleaved Caspase-3 (Asp175) Rabbit pAb (Cat. No. AP1027) (1:1000) (lower). Detection: chemiluminescence.
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