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| | Anti-Cytochrome c Mouse mAb (7H8.2C12) |
|  | Cat. No. AP1029 |
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| Host: Mouse | | Isotype: IgG2b | | Immunogen: equine cytochrome c | | Form: Liquid | | Formulation: In 50 mM sodium phosphate. | | Preservative: ≤0.1% sodium azide | | Positive Control: MCF7, P388D1, Hela, Jurkat, or NIH/3T3 cells | | Comments: Recognizes the ~15 kDa cytochrome c protein in MCF-7, P388D1, Hela, Jurkat, or NIH/3T3 cells. | | Ref.: Bossy-Wetzel, E., et al. 1998. EMBO J. 17, 37. Kluck, R.M., et al. 1997. Science 275, 1132. Yang, J., et al. 1997. Science 275, 1129. Krippner, A., et al. 1996. J. Biol. Chem. 271, 21629. Liu, X., et al. 1996. Cell 86, 147. Gonzales, D.H., et al. 1990. J. Bioenerg. Biomembr. 22, 753. Evans, M.J., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 9625. |
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| 7H8.2C12 | horse, human, mouse, rat | FC, IB, IC, NOT IP, PS
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Lane 1: Detection of human cytochrome c by immunoblotting. Samples: Whole cell lysate (50 mg) from MCF7 cells. Primary antibodies: Anti-Cytochrome c Mouse mAb (7H8.2C12) (Cat. No. AP1029) (1 mg/ml). Detection: chemiluminescence. Lanes 2,3: Detection of human cytochrome c by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 mg) from MCF7 cells. Antibody for immunoprecipitation: Anti-Cytochrome c Mouse mAb (7H8.2C12) (Cat. No. AP1029) (10 mg/ml) (lane 2) and Anti-Cytochrome c Mouse mAb (6H2.B4) (Cat. No. AP1030) (10 mg/ml) (lane 3). Immunoblotting conditions: same as lane 1.
Detection of human cytochrome c by immunocytochemistry. Sample: HeLa cells fixed with 100% methanol. Primary antibody: Anti-Cytochrome c Mouse mAb (7H8.2C12) (Cat. No. AP1029) (3 mg/ml). Detection: fluorescence with DAPI counterstain.
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