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| | Anti-PTEN Mouse mAb (EMD-4B8) |
|  | Cat. No. AP1042 |
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Anti-MMAC
Anti-Phosphatase and Tensin Homologue Deleted on Chromosome Ten
Anti-TEP-1 | Host: Mouse | | Isotype: IgG1 | | Immunogen: full-length, recombinant, human PTEN expressed in Sf9 cells | | Form: Liquid | | Formulation: In PBS, pH 7.2. | | Preservative: ≤ 0.1% sodium azide | | Positive Control: MCF7 or A431 cells | | Negative Control: MDA-MB-468 cells | | Comments: Recognizes the ~55 kDa PTEN protein in MCF-7 cells. | | Ref.: Leslie, N.R. and Downes, C.P. 2004. Biochem. J. 382, 1. Tamura, M., et al. 1998. Science 280, 1614. Steck, P.A., et al. 1997. Nat. Genetics 15, 356. |
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Detection of human PTEN by immunoblotting. Samples: Whole cell lysate (20 µg) from MCF7 cells (Lane 1), A431 cells (Lane 2), and MDA-MB-48 cells (Lanes 3,4), and PTEN, His•Tag® and S•Tag™, Human, Recombinant, S. frugiperda (20 ng; Cat. No. 481409). Primary antibody: Anti-PTEN Mouse mAb (EMD-4B8) (Cat. No. AP1042) (2 µg/ml). Detection: colorimetric.
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